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KMID : 0880220160540080559
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Journal of Microbiology
2016 Volume.54 No. 8 p.559 ~ p.564
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Amino acid residues in the Ler protein critical for derepression of the LEE5 promoter in enteropathogenic E. coli
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Choi Su-Mi
Jeong Jae-Ho
Choy Hyon-E.
Shin Min-Sang
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Abstract
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Enteropathogenic E. coli causes attaching and effacing (A/E) intestinal lesions. The genes involved in the formation of A/E lesions are encoded within a chromosomal island comprising of five major operons, LEE1-5. The global regulator H-NS represses the expression of these operons. Ler, a H-NS homologue, counteracts the H-NS?mediated repression. Using a novel genetic approach, we identified the amino acid residues in Ler that are involved in the interaction with H-NS: I20 and L23 in the C-terminal portion of α-helix 3, and I42 in the following unstructured linker region.
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KEYWORD
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enteropathogenic E. coli (EPEC), H-NS, LEE, Ler, transcription repression
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